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MCB Final Review Part 2

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4 Categories 24 Questions
Does crystal violet stain live or dead cells? What does crystal violet bind to?
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Questions
Number of categories
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5
6
Questions per category
1 2 3 4
5 6
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Category 1
Q.
Does crystal violet stain live or dead cells?
A.
Clone to My Questions
Live
Q.
What does crystal violet bind to?
A.
Clone to My Questions
DNA and proteins in the cells
Q.
What were you trying to determine in the Crystal Violet Assay?
A.
Clone to My Questions
EC50
Q.
What was the positive and negative control in the Crystal Violet assay?
A.
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Positive- H2O2Negative- Media
Q.
What is this?
A.
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Crystal Violet
Q.
Was the In Cell Analyzer experiment qualitative or quantitative?
A.
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Quantitative
Category 2
Q.
What 2 DNA intercalating dyes were used in the In Cell Analyzer experiment?
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Hoechst and Propidium Iodide
Q.
What does Hoechst stain, and why can it enter all cells?
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It stains DNA in live and dead cells because it’s hydrophobic and can pass through intact membranes.
Q.
What does Propidium Iodide (PI) stain, and why does it only enter dead cells?
A.
Clone to My Questions
PI stains DNA but is charged, so it can’t cross healthy membranes; it only enters cells with damaged membranes (dead cells).
Q.
What property of the cell does the In Cell Analyzer assay rely on to distinguish live vs. dead cells?
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Membrane integrity- Whether the membrane of the cell is intact or broken
Q.
What kind of microscope and analysis are used for this assay?
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Fluorescent microscopy combined with data segmentation software on the In-Cell Analyzer.
Q.
What is another name for the Cell Migration Assay?
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Wound Healing Assay
Category 3
Q.
What biological process does the cell migration assay model?
A.
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How cells move to close a “wound,” similar to how they migrate during cell metastasis
Q.
What do cells do to move and close the wound in the cell migration assay?
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They polarize, extend protrusions, and migrate
Q.
Why is FCS (Fetal Calf Serum) used at 5% instead of 10% in the cell migration assay?
A.
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To slow down cell proliferation, so movement (migration) not division is being measured
Q.
What are the three main steps of the wound-healing assay process?
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Gap making, image acquisition, and data analysis
Q.
What were the positive and negative controls for the In Cell Analyzer assay?
A.
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Positive- H2O2Negative- Media
Q.
•What is the color Hoechst emits? Propidium Iodide?
A.
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Hoechst- BluePropidium Iodide- Red
Category 4
Q.
Why do we wash with PBS instead of water in the cell migration assay?
A.
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PBS has the same salt balance as our cells; Water is hypotonic (having no salts or ions) and would cause the cells to explode
Q.
What were the positive and negative controls for the cell migration assay?
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Positive- CeramideNegative- Media
Q.
What was the purpose of the cell cycle assay?
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To determine what percentages of cells are in each phase of the cell cycle
Q.
What were the positive and negative controls for the cell cycle assay?
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Positive- VinblastinNegative- Media
Q.
What DNA binding dye was used for the cell cycle experiment?
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Draq5
Q.
Which compound killed 50% off the cells and at what concentration?
A.
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Capsaicin at 8.25 uM
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